针刺研究

2013, v.38(04) 291-296

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合募配穴对应激性胃溃疡大鼠下丘脑促性腺激素释放激素和P物质mRNA表达的影响
Effect of Acupuncture of Ho(ST 36)-plus Mu(CV 12)-acupoints on Hypothalamic Gonadotropin-releasing Hormone mRNA and SP mRNA Expression in Stress-induced Gastric Ulcer Rats

董莉莉;刘安国;王军燕;马重兵;朱田田;严兴科;
DONG Li-li,LIU An-guo,WANG Jun-yan,MA Chong-bing,ZHU Tian-tian,YAN Xing-ke(Department of Acu-moxibustion,Gansu College of Chinese Medicine,Lanzhou 730000,China)

摘要(Abstract):

目的:探讨下丘脑促性腺激素释放激素(GnRH)、P物质(SP)mRNA表达在合募配穴针刺防治应激性胃溃疡中的作用机制,并探讨合募配穴和单穴针刺的效应差异。方法:雄性Wistar大鼠随机分为正常组、模型组、捆绑组、中脘组、足三里组和合募配穴组,每组10只。利用捆绑加水浸应激法复制胃溃疡大鼠模型。足三里组取"足三里"穴针刺,中脘组取"中脘"穴针刺,合募配穴组取"足三里"加"中脘"穴针刺,每日1次,共治疗2次。利用RT-PCR技术观察针刺合募配穴对下丘脑GnRH mRNA、SP mRNA表达的影响。结果:捆绑组、模型组大鼠同正常组比较,溃疡指数显著升高(P<0.01);中脘组、足三里组、合募配穴组同模型组比较,溃疡指数明显下降(P<0.05,P<0.01),且以合募配穴效果更佳。捆绑组、模型组大鼠同正常组比较,下丘脑组织GnRH mRNA表达显著增加(P<0.05,P<0.01);各针刺组和模型组相比,GnRHmRNA表达均显著下降(P<0.01);各针刺组之间的差异无统计学意义(P>0.05)。捆绑组、模型组和正常组相比,下丘脑组织SP mRNA表达的差异无统计学意义(P>0.05);足三里组、合募配穴组同模型组相比,SP mRNA表达显著增加(P<0.01);合募配穴组表达的增加较足三里组更明显(P<0.05)。结论:针刺可能是通过抑制下丘脑GnRH mRNA的表达,促进下丘脑SP mRNA的表达来防治应激性胃溃疡的发生,合募配穴具有一定的特异性。
Objective To observe the effect of acupuncture of "Zusanli" and "Zhongwan"(CV 12,front Mu-acupoint) on the expression of hypothalamic gonadotropin-releasing hormone(GnRH) mRNA and SP mRNA in stress-induced gastric ulcer(GU) rats.Methods Sixty male Wistar rats were randomly divided into normal control group,simple bundling group,GU model group,CV 12 group,ST 36 group and CV 12+ST 36(Ho-and front Mu-acupoint combination) group,with 10 cases in each group.GU model was established by binding the rat's four limbs to an animal board and to immerse it to cool water for 24 h.Manual acupuncture stimulation was applied to CV 12,bilateral ST 36 or CV 12+ST 36 for 20 min,once daily for 2 days.The GU index was evaluated by using Guth's method(1979).GnRH mRNA and SP mRNA expression of the hypothalamus tissue was detected by reverse transcription-polymerase chain reaction(RT-PCR).Results Compared with the normal group,the GU index values in the simple bundling group and the model group were significantly higher(P<0.01);while in comparison with the model group,the GU index values were significantly decreased in the CV 12,ST 36 and CV 12+ST 36 groups(P<0.05,P<0.01).Following stress stimulation,hypothalamic GnRH mRNA expression levels in the simple bundling group and the model group were remarkably increased(P<0.05,P<0.01),whereas those of hypothalamic SP mRNA expression had no apparent changes in these two groups(P>0.05).After manual acupuncture intervention,hypothalamic GnRH mRNA expression levels in the CV 12,ST 36 and CV 12+ST 36 groups were evidently down-regulated and SP mRNA expression levels in the ST 36 and CV 12+ST 36 groups were remarkably upregulated in comparison with the model group(P<0.01).No significant differences were found between the CV 12 and ST 36 groups in the GU index,among the CV 12,ST 36 and CV 12+ST 36 groups in GnRH mRNA expression,and between the CV 12 and model groups in the expression levels of SP mRNA(P>0.05).Conclusion Acupuncture of CV 12 and ST 36 can relieve stress-induced gastric ulcer in the rat,which is closely with its effect in down-regulating hypothalamic GnRH mRNA expression.

关键词(KeyWords): 胃溃疡;针刺;合募配穴;下丘脑;促性腺激素释放激素mRNA;P物质mRNA
Gastric ulcer;Acupuncture;Ho-and Mu-acupoints combination;Hypothalamus;Gonadotropin-releasing hormone mRNA;SP mRNA

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目资助(No.81072863);; 教育部博士点专项科研基金资助(No.20092227120002);; 吉林省教育厅科研项目(2009第377)

作者(Authors): 董莉莉;刘安国;王军燕;马重兵;朱田田;严兴科;
DONG Li-li,LIU An-guo,WANG Jun-yan,MA Chong-bing,ZHU Tian-tian,YAN Xing-ke(Department of Acu-moxibustion,Gansu College of Chinese Medicine,Lanzhou 730000,China)

DOI: 10.13702/j.1000-0607.2013.04.008

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