王华;刘静;刘建民;吕景芳;陈明扬;王静芝;
WANG Hua,LIU Jing,LIU Jian-min,Lü Jing-fang,CHEN Ming-yang,WANG Jing-zhi(College of Acu-moxibustion and Orthopedics,Hubei University of Chinese Medicine,Wuhan 430061,China)

• 1:湖北中医药大学针灸骨伤学院

摘要(Abstract)：

目的:探讨"双固一通"电针治疗对老年阳虚模型大鼠抗疲劳能力及其肝脏线粒体呼吸功能的影响。方法:SD雄性大鼠48只,随机分为正常对照组、模型组、"双固一通"电针组、电针对照组。除正常对照组外,其余3组大鼠每日皮下注射D-半乳糖再肌肉注射氢化可的松制作老年阳虚模型。"双固一通"电针组取"关元""后三里""百会",电针对照组取"中极""阴陵泉""印堂",每次治疗15min,每周治疗6次,共4周。以游泳力竭实验潜伏期判定大鼠抗疲劳能力;采用Clark氧电极法检测肝脏线粒体的呼吸功能,包括Ⅲ态呼吸耗氧速率(R 3)、Ⅳ态呼吸耗氧速率(R 4)、呼吸控制率(RCR)以及磷氧比值(P/O)。结果:各组大鼠平均游泳力竭时间比较,模型组较正常对照组明显缩短(P<0.01),"双固一通"电针组比模型组明显延长(P<0.01),且"双固一通"电针组与电针对照组之间比较差异有统计学意义(P<0.05)。大鼠肝线粒体R 3各组间未显示明显差异;模型组R 4较正常对照组明显增加(P<0.01),"双固一通"电针组较模型组明显降低(P<0.01),且"双固一通"电针组与电针对照组之间比较差异有统计学意义(P<0.05);各组大鼠肝线粒体RCR和P/O比较,模型组两项指标较正常对照组均明显降低(P<0.01),而"双固一通"电针组两项指标较模型组均明显升高(P<0.01,P<0.05),电针对照组两项指标与"双固一通"电针组比较明显降低(P<0.05)。结论:"双固一通"配穴电针治疗能明显改善老年阳虚模型大鼠抗疲劳能力,可能与其能降低老年阳虚模型大鼠肝脏细胞线粒体R 4,提高其RCR及P/O,改善肝脏线粒体呼吸功能,继而改善机体代谢有关,且"双固一通"电针疗效优于"中极""阴陵泉""印堂"配伍电针治疗。
Objective To observe the effect of electroacupuncture(EA) of "Guanyuan"(CV 4) and bilateral "Housanli"(ST 36) plus acupuncture stimulation of "Baihui"(GV 20) [an acupoint recipe for "Shuanggu Yitong"(strengthening both congenital foundation and the acquired constitution and regulating the yang-qi of the body] on the anti-fatigue ability and liver mitochondrial respiratory function in yang-deficiency ageing rats.Methods A total of 48 male SD rats were equally and randomly divided into normal control,model,EA and EA control groups.The aged yang-deficiency model was established by subcutaneous injection of D-galactose(125 mg/kg,once daily for 40 days) and sequential muscular injection of hydrocortisone(1.5 mg/100 g,once daily for 7 days).For rats of the normal control group,subcutaneous injection of same dose of normal saline was gi-ven.EA stimulation(2 Hz,1 mA) of "Guanyuan"(CV 4) and bilateral "Housanli"(ST 36) and manual acupuncture stimulation of "Baihui"(GV 20) were given to rats of the EA group.For rats of the EA control group,EA of "Zhongji"(CV 3) and bilateral "Yinlinquan"(SP 9),and manual acupuncture stimulation of "Yintang"(EX-HN 3) were given.The treatment was conducted once daily,6 time a week and continuously for 4 weeks.Rats of the normal control and model groups were grabbed and fixed in the same way.The anti-fatigue ability was evaluated by using the time of exhausted swimming task and liver mitochondrial respiratory function was detected by Clark oxygen electrode.Results In comparison with the normal control group,the exhausted swimming time was significantly shortened in the model group(P<0.01).Compared with the model group,the exhausted swimming time was evidently prolonged in the EA group(P<0.01) not in the EA control group(P>0.05).As for the liver mitochondrial respi-ratory function,the oxygen consumption rate Ⅳ was obviously higher in the model group than in the normal control group(P<0.01),and significantly lower in the EA group than in the model group(P<0.01).Both liver mitochondrial respiratory control rate(RCR) and ratio of phospho-rus/oxgen(P/O) were significantly lower in the model group than in the normal control group(P<0.01),and considerably higher in the EA group than in the model group(P<0.01,P<0.05).Comparison between the EA and EA control groups showed that the effects of the former group were evidently superior to those of the latter group in prolonging the exhausted swimming time,lo-wering oxygen consumption rate Ⅳ and upregulating RCR and P/O(P<0.05).Conclusion Electroacupuncture of CV 4 and ST 36 plus manual acupuncture stimulation of GV 20 can improve the anti-fatigue capability in aging rats with yang-deficiency,which may be related to its effects in reducing liver mitochondrial oxygen consumption and increasing liver mitochondrial RCR and ratio of P/O.

关键词(KeyWords)： 阳虚证;老年;电针疗法;关元;后三里;百会;抗疲劳;肝脏线粒体呼吸功能
Yang-deficiency;Aging;Electroacupuncture treatment;Guanyuan(CV 4);Housanli(ST 36);Baihui(GV 20);Anti-fatigue;Liver mitochondrial respiratory function

Abstract:

Keywords:

基金项目(Foundation): 湖北省自然科学基金重点项目(No.2008CDA 051)

作者(Authors): 王华;刘静;刘建民;吕景芳;陈明扬;王静芝;
WANG Hua,LIU Jing,LIU Jian-min,Lü Jing-fang,CHEN Ming-yang,WANG Jing-zhi(College of Acu-moxibustion and Orthopedics,Hubei University of Chinese Medicine,Wuhan 430061,China)

DOI: 10.13702/j.1000-0607.2013.04.002

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